Immunophenotypic Subtyping of Leukemic Cells from Iranian Patients with Acute Lymphoblastic Leukaemia: Association to Disease Outcome

Authors

  • Abdolfattah Sarafnejad Dept. of Immunology, Medical Sciences / University of Tehran
  • Fazel Shokri Dept. of Immunology, Medical Sciences / University of Tehran | Monoclonal Antibody Research Center, Avesina Research Inst, Tehran | National Cell Bank of Iran, Pasteur Inst of Iran, Tehran, Iran
  • Hodjatallah Rabbani Cancer Center Karolinska, Karolinska Inst, Stockholm, Sweden | Dept. of Immunology, Reproductive Biotechnology Research Center, Avesina Research Inst
  • Hossein Asgarian Omran Dept. of Immunology, Medical Sciences / University of Tehran
  • Jalal Khoshnoodi Dept. of Immunology, Medical Sciences / University of Tehran
  • Mahdi Shabani Dept. of Immunology, Medical Sciences / University of Tehran
  • Mahmood Jeddi-Tehrani Cancer Center Karolinska, Karolinska Inst, Stockholm, Sweden | Monoclonal Antibody Research Center, Avesina Research Inst, Tehran
  • Mohammad Faranoush Clinic of Hematology, Iran University of Medical Sciences
  • Parvaneh Vossough Clinic of Hematology, Iran University of Medical Sciences
  • Ramzan A. Sharifian Clinic of Hematology and Oncology, Medical Sciences / University of Tehran
  • Tahereh Shahrestani Dept. of Immunology, Medical Sciences / University of Tehran
Abstract:

Background: Immunophenotypic characterization of the leukemic cells has been widely used as a tool for diagnosis, classification, stratification and prognosis of leukaemia. Objective: To investigate the immunophenotypic subtype profiles of Iranian patients with acute lymphoblastic leukemia (ALL) and its association to disease outcome. Methods: In this study, a total of 60 Iranian patients with ALL were immunophenotyped by flow cytometry using a panel of monoclonal antibodies specific for CD2, CD3, CD5, CD10, CD13, CD14, CD19, CD20, CD33, CD34, CD45, HLA-DR and TdT molecules. Results: The samples were initially categorized into T-ALL (n=9), B-ALL (n=50) and mixed lineage (n=1) based on the expression patterns of CD3 and CD19 molecules. B-ALL patients could further be classified into four subtypes, including Pro-B (n=7, 11.7%), Pre-B I (n=28, 46.7%), Pre-B II (n=13, 21.7%) and immature/mature B cells (n=2, 3.3%) on the basis of expression of CD10, CD19, CD20, HLA-DR and TdT. Clinical manifestations and laboratory findings of the patients did not reveal association with immunophenotypic sub-types of ALL, with the exception of mediastinal mass and WBC count at the time of diag-nosis which were found to be significantly higher in patients with T-ALL compared with B-ALL (p=0.001 and 0.014), respectively. Conclusion: Our results indicate that overall the immunophenotypic profile of Iranian ALL patients is similar to previous reports and it might be used for monitoring of minimal residual disease and prognosis.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

immunophenotypic subtyping of leukemic cells from iranian patients with acute lymphoblastic leukaemia: association to disease outcome

background: immunophenotypic characterization of the leukemic cells has been widely used as a tool for diagnosis, classification, stratification and prognosis of leukaemia. objective: to investigate the immunophenotypic subtype profiles of iranian patients with acute lymphoblastic leukemia (all) and its association to disease outcome. methods: in this study, a total of 60 iranian patients with ...

full text

Immunophenotypic subtyping of leukemic cells from Iranian patients with acute lymphoblastic leukaemia: association to disease outcome.

BACKGROUND Immunophenotypic characterization of the leukemic cells has been widely used as a tool for diagnosis, classification, stratification and prognosis of leukaemia. OBJECTIVE To investigate the immunophenotypic subtype profiles of Iranian patients with acute lymphoblastic leukemia (ALL) and its association to disease outcome. METHODS In this study, a total of 60 Iranian patients with...

full text

Immunophenotypic Characterization of the Leukemic B-cells from Iranian Patients with Chronic Lymphocytic Leukemia: Association between CD38 Expression and Disease Progression

Background: Patients with B-cell chronic lymphocytic leukemia (B-CLL) have hetero-geneous clinical courses, thus several biological parameters need to be added to the cur-rent clinical staging systems to predict disease outcome. Recent immunophenotypic stud-ies performed mainly in Western populations have demonstrated the prognostic value of CD38 and ZAP-70 expression in B-CLL. Objectives: To i...

full text

Expression Profile of Wnt Molecules in Leukemic Cells from Iranian Patients with Acute Myeloblastic Leukemia

Background: Wnt molecules play a key role in growth, proliferation and development of some embryonic and adult organs as well as hematopoietic stem cells. Wnt signaling pathways are aberrantly activated in many tumor types, including solid tumors and hematologic malignancies. Objective: To investigate the expression profile of a large number of Wnt genes in leukemic cells from Iranian patients ...

full text

immunophenotypic characterization of the leukemic b-cells from iranian patients with chronic lymphocytic leukemia: association between cd38 expression and disease progression

background: patients with b-cell chronic lymphocytic leukemia (b-cll) have hetero-geneous clinical courses, thus several biological parameters need to be added to the cur-rent clinical staging systems to predict disease outcome. recent immunophenotypic stud-ies performed mainly in western populations have demonstrated the prognostic value of cd38 and zap-70 expression in b-cll. objectives: to i...

full text

Immunophenotypic, cytogenetic and clinical features of 113 acute lymphoblastic leukaemia patients in China.

INTRODUCTION The analysis of immunophenotype of the leukaemic cells has been of great importance for the diagnosis, classification and prognosis of acute lymphoblastic leukaemia (ALL). MATERIALS AND METHODS One hundred and thirteen Chinese patients with ALL were immunophenotyped by fl ow cytometry and 74 cases were also subjected to karyotype analysis by G-banding technology. RESULTS Of the...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 4  issue 1

pages  15- 25

publication date 2007-03-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023